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SCAP分析<em>ñ</em> -glycosylation和贩卖人体细胞
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JoVE Journal Biologia
Analysis of SCAP N-glycosylation and Trafficking in Human Cells
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SCAP分析<em>ñ</em> -glycosylation和贩卖人体细胞

DOI:
10.3791/54709-v

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11:27 min

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November 08, 2016

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, , , , , ,
1Department of Radiation Oncology,The Ohio State University Comprehensive Cancer Center and College of Medicine

Capitoli

  • 00:05Titolo
  • 01:05Detection of Endogenous SCAP Protein in Human Cells
  • 04:11Analysis of SCAP N-glycosylation in Human Cells
  • 07:15Detection of SCAP Trafficking from ER to Golgi Using GFP-SCAP in Human Cells
  • 09:07Results: SCAP Trafficking from ER to Golgi
  • 10:43Conclusion

Summary

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我们通过使用蛋白质印迹描述了从人类细胞的膜级分的分离和样品制备用于检测SCAPÑ-glycosylation和总蛋白的改进方法。我们还引进了GFP标记法使用共聚焦显微镜监测SCAP贩卖。这种协议可以在常规生物学实验室使用。

Tags

Keywords: SCAPN-glycosylationTraffickingHuman CellsSREBPLipid MetabolismPNGaseU87 CellsCell Membrane FractionsNuclear ExtractsSDS-PAGEWestern BlotBradford Protein Assay

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