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JoVE Journal Bioengineering
Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice
 

Scaffold-supported Transplantation of Islets in the Epididymal Fat Pad of Diabetic Mice

Article DOI: 10.3791/54995-v • 11:58 min • July 23rd, 2017
July 23rd, 2017 •
*1, *1, *2, 2, 1
1Department of Biomedical Engineering, Peking University, 2Institute of Molecular Medicine, Peking University
* These authors contributed equally

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This protocol demonstrates murine islet isolation and seeding onto a decellularized scaffold. Scaffold-supported islets were transplanted into the epididymal fat pad of streptozotocin (STZ)-induced diabetic mice. Islets survived at the transplantation site and reversed the hyperglycemic condition.

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Keywords: Islet Transplantation Scaffold-supported Epididymal Fat Pad Diabetes Islet Isolation Collagenase Digestion Histopaque Gradient Tissue Engineering Beta Cell Replacement Therapy
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