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Il trattamento di droga e<em> In Vivo</em> Imaging di osteoblasti-osteoclasti interazioni in un modello di Medaka pesce osteoporosi
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Drug Treatment and In Vivo Imaging of Osteoblast-Osteoclast Interactions in a Medaka Fish Osteoporosis Model
DOI:

08:53 min

January 01, 2017

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Capitoli

  • 00:05Titolo
  • 01:05Embryo Collection and Transgenic Embryo Screening
  • 03:07Medaka-larvae Bisphosphonate Treatment
  • 04:24Live Medaka-larvae Imaging
  • 05:56Results: Representative Transgenic Marker Expression Analyses in 12 – 15 DPF Medaka-larvae
  • 07:24Conclusion

Summary

Traduzione automatica

Small laboratory fish have become popular models for bone research on the mechanisms underlying human bone disorders and for the screening of bone-modulating drugs. In this report, we describe a protocol to assess the effect of alendronate on bone cells in medaka larvae with osteoporotic lesions.

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