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Biochemistry

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JoVE Journal Biochemistry
Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay
 

Visualization of Protein-protein Interaction in Nuclear and Cytoplasmic Fractions by Co-immunoprecipitation and In Situ Proximity Ligation Assay

Article DOI: 10.3791/55218-v • 10:05 min • January 16th, 2017
January 16th, 2017 •
1, 1, 1,2
1University Children's Hospital Basel (UKBB), University of Basel, 2Department of Clinical Research, University of Basel

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Protein-protein interactions can occur in both the nucleus and the cytoplasm of a cell. To investigate these interactions, traditional co-immunoprecipitation and modern proximity ligation assay are applied. In this study, we compare these two methods to visualize the distribution of NF90-RBM3 interactions in the nucleus and the cytoplasm.

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Protein-protein Interaction Co-immunoprecipitation Proximity Ligation Assay Cellular Compartments Distribution Patterns Nucleus Cytoplasm Co-ip Pla Advantages Disadvantages Authentic Interactions Rapid Analysis Single Cell Level Hex293 Cells Cytoplasmic Extraction Solution Vortex Incubation Centrifuge
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