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植物冰结合蛋白通过冰再结晶抑制和隔离的评估使用冰亲和力纯化鉴定

JoVE Journal
Biochimica

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JoVE Journal Biochimica

Identification of Plant Ice-binding Proteins Through Assessment of Ice-recrystallization Inhibition and Isolation Using Ice-affinity Purification

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植物冰结合蛋白通过冰再结晶抑制和隔离的评估使用冰亲和力纯化鉴定

DOI:

10.3791/55302-v

•

09:43 min

•

May 05, 2017

•

Melissa Bredow, Heather E. Tomalty, Virginia K. Walker^2,3

¹Department of Biology,Queen’s University, ²Department of Biomedical and Molecular Sciences,Queen’s University, ³School of Environmental Sciences,Queen’s University

Capitoli

00:05Titolo
00:45Preparation of Native Protein Extracts for Ice-recrystalization Inhibition (IRI) Analysis
02:30Splat Assay Pre-experiment Set Up and Conducting the Splat Assay
05:32Preparation of Samples for Ice-affinity Purification
06:36Conducting Ice-affinity Purification
08:14Results: Ice-hemispheres Grown Using IBP Extracts Collected from the Freeze-tolerant Grass, L. perenne
09:15Conclusion

Summary

Traduzione automatica

本文概述冰结合蛋白的从冷冻耐受性植物通过冰再结晶抑制活性，并使用冰 - 亲和纯化天然IBPS的随后分离的评估鉴定。

Tags

Keywords: Ice-binding Proteins IBPs Plant Freeze Tolerance Ice-affinity Purification Ice-recrystallization Inhibition Cold-acclimation Plant Extract Protein Extraction Centrifugation Ice-binding Activity

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