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Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

Induction of an Inflammatory Response in Primary Hepatocyte Cultures from Mice

DOI:
10.3791/55319-v

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08:32 min

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March 10, 2017

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, , , , ,
1Katz Family Drug Discovery Center and Division of Nephrology and Hypertension, Department of Medicine,University of Miami Leonard M. Miller School of Medicine, 2Department of Cell Biology and Anatomy,University of Miami Leonard M. Miller School of Medicine

Capitoli

  • 00:05Titolo
  • 00:54Preparation for Perfusion and Liver Recovery
  • 03:23Isolation and Treatment of Cells
  • 05:23Generating cDNA from Isolated RNA by Reverse Transcription and Analysis of Cells by Quantitative PCR
  • 06:32Results: Imflammatory Response in Mouse Primary Hepatocyte Cultures
  • 07:46Conclusion

Summary

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Traduzione automatica

Here, we show an enzymatic approach to isolate primary hepatocytes from adult mice, and we describe the quantification of an inflammatory response using ELISA and real-time PCR.

Tags

HepatocytesInflammatory ResponseCytokinesCRPIL6Hepatic Acute Phase ResponseHepatocellular FunctionHepatic CytokinesChronic Kidney DiseaseHepatic MetabolomicsInflammationLiver PerfusionLiver DigestIVC CannulationLiver Dissection

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