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Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

Defined and Scalable Generation of Hepatocyte-like Cells from Human Pluripotent Stem Cells

DOI:
10.3791/55355-v

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08:36 min

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March 02, 2017

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, , , , , ,
1MRC Centre for Regenerative Medicine,University of Edinburgh

Capitoli

  • 00:05Titolo
  • 00:36Passaging Human Pluripotent Stem Cells (hPSCs)
  • 04:14Differentiating hPSCs to Hepatocyte-like Cells on Recombinant Laminins
  • 06:46Results: Superior Hepatocyte Differentiation Was Achieved on Laminin Substrates
  • 08:05Conclusion

Summary

Traduzione automatica

Traduzione automatica

The method presented here describes a scalable and good manufacturing practice (GMP)-ready differentiation system to generate human hepatocyte-like cells from pluripotent stem cells. It serves as a cost-effective and standardized system to generate human hepatocyte-like cells for basic and applied human liver research.

Tags

Human Pluripotent Stem CellsHepatocyte-like CellsDefined ConditionsRecombinant Laminin MatricesDifferentiation ProtocolCell CultureScalable ProductionBiomedical ResearchCell-based ModelingCell Therapy

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