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JoVE Journal Neuroscience
Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility
 

Rapid and Refined CD11b Magnetic Isolation of Primary Microglia with Enhanced Purity and Versatility

Article DOI: 10.3791/55364-v • 07:54 min • April 13th, 2017
April 13th, 2017 •
*1, *1, 1, 1, 1, 1, 1, 1
1Biomedical Sciences & Iowa Center for Advanced Neurotoxicology, Iowa State University
* These authors contributed equally

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Here, we present a protocol to isolate microglia from postnatal mouse pups (day 1) for in vitro experimentation. This improvised method of isolation generates both high yield and purity, a significant advantage over alternate methods that allows broad range experimentation for the purposes of elucidating microglial biology.

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CD11b Magnetic Isolation Primary Microglia Purity Versatility In Vitro Experimentation Neuroscience Neural Inflammation Technique Isolation Time Viability Decapitated Heads Micro-dissecting Scissors Meninges Brain Transfer Trypsin EDTA Incubation Growth Medium Homogenize Pipette
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