È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:05Titolo
- 01:39Transcription Run-on Reaction
- 02:36RNA Extraction
- 05:28Affinity Purification of BrUTP-labeled RNA
- 07:56Precipitation of BrUTP-labeled RNA
- 09:23Results: Rna14 is Required for Termination of Transcription of ASC1
- 11:00Conclusion
We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.
