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Genetics

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JoVE Journal Genetics
Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach
 

Analysis of Termination of Transcription Using BrUTP-strand-specific Transcription Run-on (TRO) Approach

Article DOI: 10.3791/55446-v • 12:12 min • March 12th, 2017
March 12th, 2017 •
1, 1, 1, 1, 1, 1
1Department of Biological Sciences, Wayne State University

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We describe a basic experimental approach for analysis of termination of transcription by RNA polymerase II in vivo using BrUTP by the strand-specific transcription run-on (TRO) approach in budding yeast. This protocol can be extended to study transcription termination by other RNA polymerases both in yeast and higher eukaryotes.

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Keywords: Transcription Termination BrUTP-strand-specific Transcription Run-on (TRO) RNA Polymerase Budding Yeast In Vivo Cell Permeabilization Nascent Transcripts Transcription Run-on Reaction RNA Extraction
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