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Genetics

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JoVE Journal Genetics
Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples
 

Reverse Transcription-Loop-mediated Isothermal Amplification (RT-LAMP) Assay for Zika Virus and Housekeeping Genes in Urine, Serum, and Mosquito Samples

Article DOI: 10.3791/58436-v • 08:03 min • September 14th, 2018
September 14th, 2018 •
1, 2, 2, 1,3, 1,3
1Department of Urology, Beaumont Health System, 2Foundational Sciences, College of Medicine, Central Michigan University, 3Oakland University William Beaumont School of Medicine

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This protocol provides an efficient and low-cost method to detect Zika virus or control targets in human urine and serum samples or in mosquitoes by reverse transcription loop-mediated isothermal amplification (RT-LAMP). This method does not require RNA isolation and can be done within 30 min.

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Keywords: RT-LAMP Zika Virus Urine Serum Mosquito Diagnosis Infectious Disease Dengue Chikungunya West Nile Virus Primers Sample Preparation Master Mix Isothermal Amplification
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