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JoVE Journal Genetics
An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
 

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Article DOI: 10.3791/59628-v • 09:45 min • May 21st, 2019
May 21st, 2019 •
1, 1, 1, 1
1Department of Biomedical Science and Research Institute for Bioscience & Biotechnology, Hallym University

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This protocol uses a probe-based real-time polymerase chain reaction (PCR), a sulforhodamine B (SRB) assay, 3’ untranslated regions (3’ UTR) cloning, and a luciferase assay to verify the target genes of a miRNA of interest and to understand the functions of miRNAs.

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Keywords: MicroRNA MicroRNA Target In Vitro Protocol Tumor Cells Cell Viability Assay Transfection MicroRNA Mimic MicroRNA-107
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