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Immunology and Infection

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JoVE Journal Immunology and Infection
En IL-8 Transient Transgenized Mouse Model for
 
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En IL-8 Transient Transgenized Mouse Model for

Article DOI: 10.3791/55499-v • 08:16 min • July 7th, 2017
July 7th, 2017 •
*1, *2, *3, 3, 4, 5,2, 6, 7, 2, 8, 1
1Department of Medicine, General Pathology Division, Cystic Fibrosis Translational Research Laboratory "D. Lissandrini", University of Verona, 2Corporate Preclinical R&D, Chiesi Farmaceutici S.p.A., 3Department of Diagnostic and Public Health, Microbiology Division, University of Verona, 4Dipartimento di Scienze Medico-Veterinarie, University of Parma, 5Department of Biomedical Biotechnological and Translational Sciences, University of Parma, 6Department of Computer Science, University of Verona, 7Department of Neurological, Biomedical and Movement Sciences, University of Verona, 8Cystic Fibrosis Regional Center (CFC), AOUI Verona
* These authors contributed equally

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Fremgangsmåden beskrevet her tillader visualisering af IL-8-promotorafhængig inflammationsaktivering i lungerne af mus gennem ikke-invasiv bioluminescensbilleddannelse (BLI). Det samme dyr kan udsættes for BLI flere gange i op til to måneder fra leveringstidspunktet for luciferase-reporterkonstruktionen.

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Immunology Inflammation gen delivery, bioluminescens billeddannelse interleukin-8 lunge
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