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Immunology and Infection

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JoVE Journal Immunology and Infection
Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm
 

Protocols for Investigating the Host-tissue Distribution, Transmission-mode, and Effect on the Host Fitness of a Densovirus in the Cotton Bollworm

Article DOI: 10.3791/55534-v • 11:12 min • April 12th, 2017
April 12th, 2017 •
1, 2, 3, 1, 1
1State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 2Tobacco Research Institute, Chinese Academy of Agricultural Sciences, 3Crop and Environment Sciences, Harper Adams University

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Here, we present a protocol to investigate the host-tissue distribution, transmission mode, and effect on the host fitness of a densovirus within a lepidopteran species, the cotton bollworm. This protocol can also be used for studying the interaction between other orally-transmitted viruses and their insect hosts.

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Keywords: Cotton Bollworm Densovirus HaDV2 Virus-host Interaction Virus Transmission Host Fitness PCR Genetic Background Single-pair Mating Adult Moths Egg Collection Larval Rearing Virus Detection Virus-positive Strain Virus-negative Strain
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