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Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes
 

Glucose Uptake Measurement and Response to Insulin Stimulation in In Vitro Cultured Human Primary Myotubes

Article DOI: 10.3791/55743-v • 08:03 min • June 25th, 2017
June 25th, 2017 •
1, 1, 1, 2, 3, 1, 1
1CarMeN Laboratory, INSERM U1060, INRA 1397, University of Lyon, 2Department of digestive and bariatric surgery, Obesity Integrated Center, University Hospital of Edouard Herriot, Hospices Civils de Lyon, Lyon 1 University, 3Division of Endocrinology, Diabetes, Hypertension and Nutrition, Department of Clinical Medicine, Faculty of Medicine, University of Geneva

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In this method, human primary muscle cells are cultured in vitro to obtain differentiated myotubes and glucose uptake rates are measured. We provide a detailed protocol to quantify rates in basal and insulin-stimulated states using radiolabeled [3H] 2-deoxy-D-Glucose.

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Glucose Uptake Measurement Insulin Stimulation In Vitro Cultured Human Primary Myotubes Muscle Metabolism Insulin Sensitivity Biological Effect Proliferation Medium PM Fetal Calf Serum FCS Serum Substitute Glutamine Penicillin Streptomycin Differentiation Medium DM Dulbecco's Modified Eagle Medium DMEM X Dulbecco's Phosphate Buffered Saline XDPBS Bovine Serum Albumen Cold 2DG Solution Radiolabeled 2DG
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