Rivista
/
Biologia dello sviluppo
/
通过显微注射卵母细胞生成转基因小鼠
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Generation of Genetically Modified Mice through the Microinjection of Oocytes
Please note that all translations are automatically generated. Click here for the English version.

通过显微注射卵母细胞生成转基因小鼠

DOI:
10.3791/55765-v

•

10:19 min

•

June 15, 2017

•

,
1Transgenic Animal Unit, Mark Wainwright Analytical Centre,University of New South Wales

Capitoli

  • 00:05Titolo
  • 00:53Synthesis of CRISPER Components
  • 04:21Injection Mix and Pronuclear and Cytoplasmic Injections
  • 06:31Reimplantation
  • 08:21Results: Microinjection of Oocytes to Generate Genetically Modified Mice
  • 09:49Conclusion

Summary

Traduzione automatica

Traduzione automatica

小鼠卵母细胞的显微注射通常用于经典转基因（ 即转基因的随机整合）和CRISPR介导的基因靶向。该协议审查显微注射的最新发展，特别强调质量控制和基因分型策略。

Tags

AI-generated MiceCRISPR-Cas9 Gene EditingOocyte MicroinjectionTransgenic Mouse ProductionRNA SynthesisIn Vitro TranscriptionDNA Purification

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/55765?article_type=v

通过成熟的脂肪细胞在天花板文化去分化代人脂肪干细胞
check_url/it/55765?article_type=v

转基因器官皮肤培养使用灭活人真皮代
check_url/it/55765?article_type=v

技术目标显微注射到显影<em>爪</em>肾
check_url/it/55765?article_type=v

胚胎显微注射和电的脊索动物<em>玻璃海鞘</em
check_url/it/55765?article_type=v

从脐带血源性多能干细胞中提取三维皮肤有机细胞
check_url/it/55765?article_type=v

小鼠肝外胆管有机体的产生
check_url/it/55765?article_type=v

生成基于人类 iPSC 的血脑屏障芯片
check_url/it/55765?article_type=v

未成熟费林卵母细胞的最小体积玻璃化
check_url/it/55765?article_type=v

斑马鱼卵巢卵巢中卵泡细胞和卵母细胞的分离
check_url/it/55765?article_type=v

患者外周血单核细胞中人心肌细胞的生成和扩增

Read Article