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在慢性淋巴细胞性白血病患者中使用甲基CpG结合域捕获法进行综合DNA甲基化分析
JoVE Journal
Genetica
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JoVE Journal
Genetica
Comprehensive DNA Methylation Analysis Using a Methyl-CpG-binding Domain Capture-based Method in Chronic Lymphocytic Leukemia Patients
Please note that all translations are automatically generated.
Click here for the English version.
在慢性淋巴细胞性白血病患者中使用甲基CpG结合域捕获法进行综合DNA甲基化分析
DOI:
10.3791/55773-v
•
13:21 min
•
June 16, 2017
•
Santhilal Subhash
,
Meena Kanduri
1
Department of Medical Genetics, Institute of Biomedicine
,
Gothenburg University
,
2
Department of Clinical Chemistry and Transfusion Medicine, Institute of Biomedicine
,
Gothenburg University
Capitoli
00:05
Titolo
00:49
Genomic DNA Extraction and Sonication
02:06
Bead Preparation Prior to Binding with Methyl-CpG-binding Domain (MBD)-biotin Protein
03:35
Binding the MBD-biotin Protein to the Washed Beads
04:59
Binding MBD-biotin Beads with Fragmented Genomic DNA
06:22
Removing the Unbound DNA and Eluting the Methylated DNA from the Beads
08:10
Ethanol Precipitation and the Enrichment of Methylated DNA
10:09
Results: Identification of Differentially Methylated CpG Rich Regions in Chronic Lymphocytic Leukemia (CLL) Samples
11:26
Conclusion
Summary
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该工作描述了优化的甲基CpG结合结构域(MBD)测序方案和用于鉴定慢性淋巴细胞性白血病(CLL)患者中差异甲基化CpG富集区的计算管道。
Tags
DNA Methylation
Methyl-CpG-binding Domain
Capture-based Method
Chronic Lymphocytic Leukemia
Next-generation Sequencing
Global Methylation Profiling
Differentially Methylated CpG-rich Regions
CLL-specific Signature Genes
Disease Pathogenesis
Prognosis
Genomic DNA Extraction
Sonication
Magnetic Streptavidin Beads
MBD-biotin Protein
Article
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