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Immunology and Infection

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JoVE Journal Immunology and Infection
Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells
 

Induction of Cellular Differentiation and Single Cell Imaging of Vibrio parahaemolyticus Swimmer and Swarmer Cells

Article DOI: 10.3791/55842-v • 08:38 min • May 15th, 2017
May 15th, 2017 •
1, 1, 1
1Department of Ecophysiology, Max Planck Institute for Terrestrial Microbiology

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This protocol enables single cell microscopy of the differentially distinct Vibrio parahaemolyticus swimmer and swarmer cells. The method produces a population of swarmer cells easily available for single cell analysis and covers preparation of cell cultures, induction of swarmer differentiation, sample preparation, and image analysis.

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Keywords: Vibrio Parahaemolyticus Cellular Differentiation Swarmer Cells Single Cell Imaging Fluorescence Microscopy Cell Cycle Cell Morphology Intracellular Organization 2,2'-Bipyridyl Calcium Chloride Heart Infusion Agar
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