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Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling
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JoVE Journal Chimica
Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

Chemo-enzymatic Synthesis of N-glycans for Array Development and HIV Antibody Profiling

DOI:
10.3791/55855-v

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11:08 min

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February 05, 2018

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, , ,
1Genomics Research Center,Academia Sinica

Capitoli

  • 00:04Titolo
  • 01:23Preparation of Glycans with Phosphonic Acid Tail
  • 04:12Glycan Array
  • 08:18Results: Synthesis of N-glycans and Development of Arrays for HIV Antibody Profiling
  • 09:59Conclusion

Summary

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A modular approach to the synthesis of N-glycans for attachment to an aluminum oxide-coated glass slide (ACG slide) as a glycan microarray has been developed and its use for the profiling of an HIV broadly neutralizing antibody has been demonstrated.

Tags

Chemo-enzymatic SynthesisN-glycansGlycan MicroarrayHIV Antibody ProfilingGlycobiologyCell Virus Specific GlycansGlycoarrayInfluenza DiagnosisCancer DiagnosisSerum AntibodiesGlycan SpecificitiesPhosphonic Acid LinkerPolyacrylamide GelTLCCerium Ammonium MolybdatePalladium HydroxideHydrogen Atmosphere

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