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Cancer Research

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JoVE Journal Cancer Research
Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model
 

Identification and Characterization of Metastatic Factors by Gene Transfer into the Novel RIP-Tag; RIP-tva Murine Model

Article DOI: 10.3791/55890-v • 09:03 min • October 16th, 2017
October 16th, 2017 •
1, 1, 1
1Department of Pathology and Laboratory Medicine, Weill Cornell Medicine

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We present a protocol to demonstrate a novel somatic gene transfer system utilizing RIP-Tag; RIP-tva mouse model to study the function of genes in metastasis. The avian retroviruses are delivered intracardiacally to ensure gene transfer into pre-malignant, noninvasive lesions of pancreatic β cells in adult mice.

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Keywords: Metastatic Factors Gene Transfer RIP-Tag; RIP-tva Mouse Model Pancreatic Beta Cell Precursor Lesions Virus Producing DF1 Cells Viral Supernatant Ultracentrifugation Viral Titer Serial Dilution DF1 Cells
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