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实验模型研究酸性后处理对脑缺血的神经保护作用

JoVE Journal
Neuroscienze

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JoVE Journal Neuroscienze

Experimental Models to Study the Neuroprotection of Acidic Postconditioning Against Cerebral Ischemia

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实验模型研究酸性后处理对脑缺血的神经保护作用

DOI:

10.3791/55931-v

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10:13 min

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July 31, 2017

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Yanrong Zheng*¹, Zhe Shen*¹, Xiaoli Wu, Lei Jiang, Weiwei Hu, Zhong Chen, Xiangnan Zhang

¹Institute of Pharmacology & Toxicology, College of Pharmaceutical Sciences, Department of Pharmacology, Key Laboratory of Medical Neurobiology of The Ministry of Health of China, Zhejiang Province Key Laboratory of Neurobiology,Zhejiang University

Capitoli

00:05Titolo
00:57Cortical Slice Preparation
02:43Oxygen-glucose Deprivation and Acidic Postconditioning
04:03Determination of Slice Viability
05:04Middle Cerebral Artery Occlusion
07:22Reperfusion and Acidic Postconditioning
08:16Results: Acidic Preconditioning Protects Against Reperfusion Injury
09:32Conclusion

Summary

Traduzione automatica

酸性后处理可防止脑缺血。在这里我们提出两个模型来执行APC。分别通过体外氧葡萄糖剥夺后将皮质硬膜下切片转移到酸性缓冲液中，并在体内大脑中动脉闭塞后吸入20％CO ₂ 。

Tags

Keywords: Neuroprotection Acidic Postconditioning Cerebral Ischemia Cortico Striatal Slice Model Middle Cerebral Artery Occlusion Model Ischemic Brain Injury Stroke Therapy Acidosis Postconditioning Brain Slice Preparation Glucose-free ACSF Acidic ACSF Synaptic Function Recovery

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