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阻力动脉的细胞培养模型
JoVE Journal
Medicina
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JoVE Journal Medicina
A Cell Culture Model of Resistance Arteries
DOI:

10:54 min

September 08, 2017

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Capitoli

  • 00:05Titolo
  • 00:49Plating Cells for Vascular Cell co-culture
  • 04:14Harvesting Fractions from the VCCC
  • 07:21Harvesting and Embedding the VCCC for Transverse Immunofluorescence
  • 09:11Results: Myoendothelial Junction Formation in Vascular Cell Co-cultures
  • 10:18Conclusion

Summary

Traduzione automatica

一个细胞培养模型的阻力动脉描述, 允许解剖的信号通路的内皮, 平滑肌, 或之间的内皮和平滑肌 (myoendothelial 交界处)。选择性应用激动剂或蛋白质隔离, 电子显微镜, 或免疫荧光可以利用这种细胞培养模型。

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