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真菌细胞的活细胞成像研究抗真菌植物防御的进入和亚单位定位
JoVE Journal
Biologia
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JoVE Journal Biologia
Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
DOI:

08:39 min

December 24, 2017

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Capitoli

  • 00:05Titolo
  • 00:57Preparing Conidial Suspensions for Imaging
  • 03:30Subcellular Localization of MtDef4 Defensin into Fungal Cells
  • 04:55Time Lapse Imaging of Fungi to Track the Internalization of MtDef5 Defensin
  • 06:31Results: Trafficking of MtDef4 and MtDef5 N. Crassa and F. graminearum
  • 07:47Conclusion

Summary

Traduzione automatica

植物防御在植物防御病原体中起着重要的作用。为了有效地使用这些抗真菌肽作为抗真菌剂, 了解其作用方式 (MOA) 是至关重要的。在这里, 描述了一个活细胞成像方法, 以研究这些肽的 MOA 的关键方面。

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