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Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
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Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins

DOI:
10.3791/55995-v

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08:39 min

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December 24, 2017

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, ,
1Donald Danforth Plant Science Center, 2Department of Biology,Kutztown University of Pennsylvania

Chapters

  • 00:05Title
  • 00:57Preparing Conidial Suspensions for Imaging
  • 03:30Subcellular Localization of MtDef4 Defensin into Fungal Cells
  • 04:55Time Lapse Imaging of Fungi to Track the Internalization of MtDef5 Defensin
  • 06:31Results: Trafficking of MtDef4 and MtDef5 N. Crassa and F. graminearum
  • 07:47Conclusion

Summary

Automatic Translation

Automatic Translation

Plant defensins play an important role in plant defense against pathogens. For effective use of these antifungal peptides as antifungal agents, understanding their modes of action (MOA) is critical. Here, a live-cell imaging method is described to study critical aspects of the MOA of these peptides.

Tags

Keywords: Live-cell ImagingFungal CellsAntifungal Plant DefensinsConfocal MicroscopyModes Of ActionInternalizationSubcellular LocalizationFusarium GraminearumNeurospora CrassaConidia SuspensionGermlings Preparation

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