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真菌细胞的活细胞成像研究抗真菌植物防御的进入和亚单位定位
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Biology
Live-cell Imaging of Fungal Cells to Investigate Modes of Entry and Subcellular Localization of Antifungal Plant Defensins
Please note that all translations are automatically generated.
Click here for the English version.
真菌细胞的活细胞成像研究抗真菌植物防御的进入和亚单位定位
DOI:
10.3791/55995-v
•
08:39 min
•
December 24, 2017
•
Kazi T. Islam
,
Dilip M. Shah
,
Kaoutar El-Mounadi
1
Donald Danforth Plant Science Center
,
2
Department of Biology
,
Kutztown University of Pennsylvania
Chapters
00:05
Title
00:57
Preparing Conidial Suspensions for Imaging
03:30
Subcellular Localization of MtDef4 Defensin into Fungal Cells
04:55
Time Lapse Imaging of Fungi to Track the Internalization of MtDef5 Defensin
06:31
Results: Trafficking of MtDef4 and MtDef5
N. Crassa
and
F. graminearum
07:47
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
植物防御在植物防御病原体中起着重要的作用。为了有效地使用这些抗真菌肽作为抗真菌剂, 了解其作用方式 (MOA) 是至关重要的。在这里, 描述了一个活细胞成像方法, 以研究这些肽的 MOA 的关键方面。
Tags
Live-cell Imaging
Fungal Cells
Antifungal Plant Defensins
Confocal Microscopy
Modes Of Action
Internalization
Subcellular Localization
Fusarium Graminearum
Neurospora Crassa
Conidia Suspension
Germlings Preparation
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