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Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples
 

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

Article DOI: 10.3791/55031-v • 10:57 min • February 3rd, 2017
February 3rd, 2017 •
1, 2, 3, 4, 3
1Biodiversity and Conservation Department, Real Jardín Botánico, CSIC, 2Research Support Unit, Real Jardín Botánico, CSIC, 3Mycology Department, Real Jardín Botánico, CSIC, 4Division of Glycoscience, AlbaNova University Center, Royal Institute of Technology (KTH)

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Summary
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Problems in the processing of biological samples for scanning electron microscopy observation include cell collapse, treatment of samples from wet microenvironments and cell destruction. Low-cost and relatively rapid protocols suited for preparing challenging samples such as floral meristems, oomycete cysts, and fungi (Agaricales) are compiled and detailed here.

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Keywords: Scanning Electron Microscopy (SEM) SEM Protocols Plant Samples Oomycete Samples Fungal Samples Fixation FAA Fixative 70% Ethanol Formaldehyde Acetic Acid Tissue Preparation Tissue Dissection Dehydration Critical Point Drying Sample Mounting SEM Sample Holders
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