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Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)
JoVE Journal
Biology
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JoVE Journal Biology
Preparation of Prokaryotic and Eukaryotic Organisms Using Chemical Drying for Morphological Analysis in Scanning Electron Microscopy (SEM)
DOI:

09:58 min

January 07, 2019

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Chapters

  • 00:04Title
  • 00:45Fixation, Washing and Dehydration of Cyanobacteria and Euglenoids
  • 02:36Drying and Mounting
  • 03:54Fixation, Washing and Dehydration of Drosophila
  • 04:55Drosophila Drying and Mounting
  • 06:48Sputter Coating and Imaging
  • 07:20Results: Morphological Analysis of Different Organisms Using SEM Images
  • 09:20Conclusion

Summary

Automatic Translation

SEM analysis is an effective method to aid in species identification or phenotypic discrimination. This protocol describes the methods for examining specific morphological details of three representative types of organisms and would be broadly applicable to examining features of many organismal and tissue types.

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