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Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy
 

Tandem High-pressure Freezing and Quick Freeze Substitution of Plant Tissues for Transmission Electron Microscopy

Article DOI: 10.3791/51844-v • 12:52 min • October 13th, 2014
October 13th, 2014 •
1, 2, 1
1Department of Biochemical, Cellular and Molecular Biology, University of Tennessee, Knoxville, 2Advanced Microscopy and Imaging Facility, University of Tennessee, Knoxville

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Obtaining high-quality transmission electron microscopy images is challenging, especially in the case of plant cells, which have abundant large water-filled vacuoles and aerated spaces. Tandem high-pressure freezing and quick freeze substitution greatly reduce preparation time of plant samples for TEM while producing samples with excellent ultrastructural preservation.

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Keywords: High-pressure Freezing Quick Freeze Substitution Transmission Electron Microscopy Plant Tissues Cryofixation Ultrastructure Freeze Substitution Epoxy Resins Artifact-free Samples Water-filled Vacuoles Cell Walls
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