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Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice
 

Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

Article DOI: 10.3791/56018-v • 11:25 min • February 10th, 2018
February 10th, 2018 •
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1National Institute of Immunology

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We have described a protocol for performing partial hepatectomy (PHx) and cell transplantation via spleen in NOD.SCID (NOD.CB17-Prkdcscid/J) mice. In this protocol, an incision is made to expose and resect the left lobe of the liver followed by another incision for the intrasplenic transplantation of cells.

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Keywords: Hepatocyte Transplantation Partial Hepatectomy NOD.SCID Mice Cell Engraftment Cell-based Therapy GFP Mouse NeoHep Cells Isoflurane Anesthesia Surgical Procedure
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