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Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System
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Neuroscienze
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JoVE Journal Neuroscienze
Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

Live Imaging of Primary Cerebral Cortex Cells Using a 2D Culture System

DOI:
10.3791/56063-v

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10:12 min

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August 09, 2017

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, , , ,
1Brain Institute,Federal University of Rio Grande do Norte, 2Department of Biosystems Science and Engineering,ETH Zurich, 3The Solomon H. Snyder Department of Neuroscience,Johns Hopkins University

Capitoli

  • 00:05Titolo
  • 00:57Dorsolateral Telencephalon Microdissection and Cell Plating
  • 04:23Time-lapse Video Microscopy
  • 05:24Cell Tracking
  • 08:12Results: Cell Lineage of Single Progenitor Cells
  • 09:41Conclusion

Summary

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Live imaging is a powerful tool to study cellular behaviors in real time. Here, we describe a protocol for time-lapse video-microscopy of primary cerebral cortex cells that allows a detailed examination of the phases enacted during the lineage progression from primary neural stem cells to differentiated neurons and glia.

Tags

Live ImagingPrimary Cerebral Cortex Cells2D Culture SystemNeural Stem CellsNeuronsGlial CellsNeurodevelopmentCell LineageCell DivisionCell CycleCell GrowthEmbryonic BrainDorsolateral TelencephalonCell DissociationTrypsin-EDTAProliferation MediumPoly-D-lysine

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