<em>In Vivo</em> Detection and Analysis of Rb Protein SUMOylation in Human Cells

Fengxi Meng; Xiaofeng Li; Hui Ren; Jiang Qian

doi:10.3791/56096

Rivista

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Biologia

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在体内人细胞 Rb 蛋白 sumo 的检测与分析

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Biologia

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JoVE Journal Biologia

In Vivo Detection and Analysis of Rb Protein SUMOylation in Human Cells

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在体内人细胞 Rb 蛋白 sumo 的检测与分析

DOI:

10.3791/56096-v

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09:40 min

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November 02, 2017

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Fengxi Meng*^1,2, Xiaofeng Li*^1,2, Hui Ren², Jiang Qian²

¹Department of Ophthalmology,Eye and ENT Hospital of Fudan University, ²Shanghai Key Laboratory of Visual Impairment and Restoration,Fudan University

Capitoli

00:05Titolo
00:54Cell Culture and Cell Cycle Synchronization
03:18Immunoprecipitation of Endogenerous Rb Protein
06:12Analysis of 6XHis-tagged Exogenous Rb SUMOylation in Human Cells
08:20Results: Detection of Both Endogenous and Exogenous Rb SUMOylation
09:08Conclusion

Summary

Traduzione automatica

小泛素相关的修饰剂 (相扑) 家族蛋白被共轭到靶蛋白的赖氨酸残留物来调节各种细胞过程。本文介绍了一种检测视网膜母细胞瘤 (Rb) 蛋白 sumo 的方法。

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