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Horizontal Whole Mount: A Novel Processing and Imaging Protocol for Thick, Three-dimensional Tissue Cross-sections of Skin
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Capitoli
- 00:05Titolo
- 00:55Preparation of Skin Samples
- 02:07Cryosectioning
- 03:17Immunofluorescent Labeling
- 05:05Mounting for Microscopic Visualization
- 06:36Results: Horizontal Whole Mount Compared to a Classical Cryosection
- 07:55Conclusion
This work presents a novel processing and imaging protocol for thick, three-dimensional tissue cross-section analysis that enables the full exploitation of confocal imaging modalities. This protocol preserves antigenicity and represents a robust system to analyze skin histology and potentially other tissue types.
