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Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina

DOI:
10.3791/56113-v

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12:28 min

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November 10, 2017

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, , , , , , , ,
1Center for Microscopy and Image Analysis,University of Zurich, 2Institute for Molecular Life Sciences,University of Zurich, 3Institute for Medical Genetics,University of Zurich

Capitoli

  • 00:05Titolo
  • 01:12Gelatin Infiltration, Mounting, and Cryosectioning
  • 03:47Immunolabeling
  • 05:26Super-resolution Microscopy
  • 07:25Platinum Shadowing and Scanning Electron Microscopy
  • 08:48Alignment of Light and Electron Microscopy Images
  • 10:23Results: Tom20 Protein Expression in the Zebrafish Retina
  • 11:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the necessary steps to obtain subcellular protein localization results on zebrafish retina by correlating super-resolution light microscopy and scanning electron microscopy images.

Tags

Keywords: Super-resolution MicroscopyElectron MicroscopyProtein LocalizationZebrafish RetinaTokuyasu SectionsPlatinum ShadowingGelatin EmbeddingSucrose CryoprotectionCryo-ultramicrotomy

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