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斑马鱼视网膜蛋白定位的相关超分辨和电镜观察
JoVE Journal
Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Correlative Super-resolution and Electron Microscopy to Resolve Protein Localization in Zebrafish Retina
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斑马鱼视网膜蛋白定位的相关超分辨和电镜观察

DOI:
10.3791/56113-v

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12:28 min

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November 10, 2017

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, , , , , , , ,
1Center for Microscopy and Image Analysis,University of Zurich, 2Institute for Molecular Life Sciences,University of Zurich, 3Institute for Medical Genetics,University of Zurich

Capitoli

  • 00:05Titolo
  • 01:12Gelatin Infiltration, Mounting, and Cryosectioning
  • 03:47Immunolabeling
  • 05:26Super-resolution Microscopy
  • 07:25Platinum Shadowing and Scanning Electron Microscopy
  • 08:48Alignment of Light and Electron Microscopy Images
  • 10:23Results: Tom20 Protein Expression in the Zebrafish Retina
  • 11:29Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议描述了在斑马鱼视网膜上获得亚细胞蛋白定位结果的必要步骤, 方法是将超分辨光显微镜和扫描电子显微镜图像关联起来。

Tags

Super-resolution MicroscopyElectron MicroscopyProtein LocalizationZebrafish RetinaTokuyasu SectionsPlatinum ShadowingGelatin EmbeddingSucrose CryoprotectionCryo-ultramicrotomy

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