Rivista
/
Biologia
/
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
JoVE Journal
Biologia
Author Produced
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles

DOI:
10.3791/56179-v

•

06:43 min

•

March 31, 2018

•

, ,
1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED),University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva

Capitoli

  • 00:00Titolo
  • 00:32Fixation and resin embedding
  • 02:53Trimming and sectioning of blocks
  • 03:45Staining IFM sections
  • 04:06Mounting IFM sections
  • 04:26Acquisition of images and quantification of muscle area
  • 05:10Representative results
  • 06:05Conclusions

Summary

Traduzione automatica

Traduzione automatica

We report a method to quantify muscle area, which is an indirect method to determine muscle mass in Drosophila adults. We demonstrate the application of our methodology by analyzing the indirect flight muscles in a Drosophila model of Myotonic Dystrophy disease.

Tags

Drosophila MelanogasterOptical Cross-sectional Muscle AreaIndirect Flight MusclesMuscle MassMuscle AtrophyDorsal Longitudinal MusclesFixationResin EmbeddingAnesthesiaDehydrationPropylene OxideResin PolymerizationTrimmingSectioningUltramicrotome

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Dissection of Larval CNS in Drosophila Melanogaster

High-Resolution Video Tracking of Locomotion in Adult Drosophila Melanogaster

Visually Mediated Odor Tracking During Flight in Drosophila

Dissection of Oenocytes from Adult Drosophila melanogaster

Paraffin-Embedded and Frozen Sections of Drosophila Adult Muscles

Isolation of Drosophila melanogaster Testes

Insulin Injection and Hemolymph Extraction to Measure Insulin Sensitivity in Adult Drosophila melanogaster

Ex Vivo Assessment of Contractility, Fatigability and Alternans in Isolated Skeletal Muscles

Measurement of Lifespan in Drosophila melanogaster

Maintenance of a Drosophila melanogaster Population Cage

Read Article