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果蝇成人间接飞行肌的光学横截肌面积测定
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Biologia
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JoVE Journal Biologia
Optical Cross-Sectional Muscle Area Determination of Drosophila Melanogaster Adult Indirect Flight Muscles
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果蝇成人间接飞行肌的光学横截肌面积测定

DOI:
10.3791/56179-v

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06:43 min

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March 31, 2018

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, ,
1Department of Genetics and Interdisciplinary Research Structure for Biotechnology and Biomedicine (BIOTECMED),University of Valencia, 2Incliva Health Research Institute, 3Joint unit CIPF-Incliva

Capitoli

  • 00:00Titolo
  • 00:32Fixation and resin embedding
  • 02:53Trimming and sectioning of blocks
  • 03:45Staining IFM sections
  • 04:06Mounting IFM sections
  • 04:26Acquisition of images and quantification of muscle area
  • 05:10Representative results
  • 06:05Conclusions

Summary

Traduzione automatica

Traduzione automatica

我们报告的方法, 以量化肌肉面积, 这是一个间接的方法来确定肌肉质量在果蝇成人。我们通过分析强直营养不良症的果蝇模型中的间接飞行肌肉来证明我们的方法的应用。

Tags

Drosophila MelanogasterOptical Cross-sectional Muscle AreaIndirect Flight MusclesMuscle MassMuscle AtrophyDorsal Longitudinal MusclesFixationResin EmbeddingAnesthesiaDehydrationPropylene OxideResin PolymerizationTrimmingSectioningUltramicrotome

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