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Neuroscience

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JoVE Journal Neuroscience
Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy
 

Visualization and Live Imaging of Oligodendrocyte Organelles in Organotypic Brain Slices Using Adeno-associated Virus and Confocal Microscopy

Article DOI: 10.3791/56237-v • 08:38 min • October 23rd, 2017
October 23rd, 2017 •
1, 1,2
1Division of Anatomy, Institute of Basic Medical Sciences, University of Oslo, 2Department of Microbiology, Oslo University Hospital

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Myelinating oligodendrocytes promote rapid action potential propagation and neuronal survival. Described here is a protocol for oligodendrocyte-specific expression of fluorescent proteins in organotypic brain slices with subsequent time-lapse imaging. Further, a simple procedure for visualizing unstained myelin is presented.

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Visualization Live Imaging Oligodendrocyte Organelles Adeno-associated Virus Confocal Microscopy Viral Transduction Time-lapse Recording Mitochondria Movement Local Environment Changes Oligodendrocyte Field Oligodendrocyte Structure Organelle Dynamics Brain Cells Compact Myelin Slice Cultures Diluted AAV Pipette Tip Cortex Area Fluorescent Markers Imaging Bath
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