全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

JoVE Journal
Biologia dello sviluppo

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JoVE Journal Biologia dello sviluppo

Visualizing Multiciliated Cells in the Zebrafish Through a Combined Protocol of Whole Mount Fluorescent In Situ Hybridization and Immunofluorescence

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全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

DOI:

10.3791/56261-v

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09:33 min

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November 18, 2017

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Amanda N. Marra, Marisa Ulrich, Audra White, Meghan Springer, Rebecca A. Wingert

¹Department of Biological Sciences,University of Notre Dame

Capitoli

00:05Titolo
00:50Embryo Fixation and Hybridization
03:05Hot Washes, Blocking, Antibody Incubation, and Maleic Acid Buffer Washes
04:27Probe Detection, Peroxidase Removal, and Immunofluorescence
07:19Zebrafish Pronephros Mounting and Imaging
08:23Results: Representative Zebrafish Pronephros and Multiciliated Cell (MCC) Imaging
09:09Conclusion

Summary

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纤毛发育对正常器官的形成至关重要。该协议描述了一种优化的方法来标记和可视化纤毛细胞的斑马鱼。

全贴装荧光原位杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化

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Summary

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