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小鼠外周动脉疾病模型中单核细胞归巢和肿瘤相关血管生成的活体显微镜观察

JoVE Journal
Medicina
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JoVE Journal Medicina

Intravital Microscopy of Monocyte Homing and Tumor-Related Angiogenesis in a Murine Model of Peripheral Arterial Disease

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小鼠外周动脉疾病模型中单核细胞归巢和肿瘤相关血管生成的活体显微镜观察

DOI:

10.3791/56290-v

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08:38 min

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August 26, 2017

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Martin Wagner, Claudia Baer, Werner Zuschratter, Monika Riek-Burchardt, Christian Deffge, Soenke Weinert, Jerry C Lee, Ruediger C Braun-Dullaeus, Joerg Herold

¹Department of Cardiology and Angiology,University of Magdeburg, ²Leibniz Institute for Neurobiology, ³Institute of Molecular and Clinical Immunology,University of Magdeburg, ⁴Division of Hematology/Oncology,Boston Children’s Hospital, Harvard Medical School

Capitoli

00:00Titolo
00:39Cell Preparation
01:54Anesthesia
02:00Implantation of Basement Membrane-like Matrix
03:35Tail Vein Injection
05:04Intravital Microscopy
06:07Representative Results
07:27Conclusion

Summary

Traduzione automatica

单核细胞是外周动脉疾病中 arteriogenesis 的重要介质。本协议采用基底膜样基质和活体显微镜, 研究单核细胞注射在股动脉结扎小鼠模型中的单核细胞归巢和肿瘤相关血管生成。

Tags

Intravital Microscopy Monocyte Homing Tumor-related Angiogenesis Peripheral Arterial Disease Cell Labeling Matrigel VEGF BFGF Heparin Subcutaneous Injection Systemic Cell Application

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