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Cancer Research

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JoVE Journal Cancer Research
Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
 

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines

Article DOI: 10.3791/56311-v • 06:00 min • September 13th, 2017
September 13th, 2017 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Osteoncology and Rare Tumors Center, Istituto Scientifico Romagnolo per lo Studio e la Cura dei Tumori (IRST), IRCCS

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This protocol describes development of an in vitro human preclinical model of osteoclastogenesis from peripheral blood monocytes cultured with breast cancer cell lines to mimic the cancer cell-osteoclast interaction. The model could be used to further our understanding of bone metastasis formation and improve therapeutic options.

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Human Preclinical Model Osteoclastogenesis Peripheral Blood Monocytes Breast Cancer Cell Lines Cancer Cell Osteoclast Interactions Bone Metastasis Bone Microenvironment Fully Human Pre-clinical Model Bone-related Pathological Mechanisms Osteoporosis PBMC Cell Density PBMC Selection And Seeding Steps Manual Experience Healthy-donor Whole Blood EDTA PBS Lymphocyte Separation Medium Density Gradient Centrifugation
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