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Cancer Research

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JoVE Journal Cancer Research
Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia
 

Flow Cytometric Analysis of Mitochondrial Reactive Oxygen Species in Murine Hematopoietic Stem and Progenitor Cells and MLL-AF9 Driven Leukemia

Article DOI: 10.3791/59593-v • 09:44 min • September 5th, 2019
September 5th, 2019 •
1, 1
1Blood Cell Development and Function Program, Fox Chase Cancer Center

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We describe a method for using multiparameter flow cytometry to detect mitochondrial reactive oxygen species (ROS) in murine healthy hematopoietic stem and progenitor cells (HSPCs) and leukemia cells from a mouse model of acute myeloid leukemia (AML) driven by MLL-AF9.

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Keywords: Flow Cytometry Mitochondrial Reactive Oxygen Species Hematopoietic Stem Cells Hematopoietic Progenitor Cells Acute Myeloid Leukemia MLL-AF9 Redox State Metabolism
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