Rivista
/
/
持续药物输注系统对小鼠脑内下丘脑胰岛素信号的研究
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Studying the Hypothalamic Insulin Signal to Peripheral Glucose Intolerance with a Continuous Drug Infusion System into the Mouse Brain
DOI:

08:32 min

January 04, 2018

,

Capitoli

  • 00:05Titolo
  • 01:21Preparation of Micro-osmotic Pump Infusion Systems
  • 02:41Intracerebral Ventricular Surgery – Implantation of the Micro-osmotic Pump
  • 04:15Oral Glucose Tolerance Test (OGTT) and Insulin Tolerance Test (ITT)
  • 06:04Results: Glucose Metabolism and Insulin Signal Activation
  • 07:27Conclusion

Summary

Traduzione automatica

该协议研究的作用趋化因子 (c-c 主题) 配体 5 (CCL5) 在下丘脑通过提供拮抗剂,满足CCL5, 进入鼠标的大脑使用微渗透泵脑输液系统。这种瞬态抑制 CCL5 活动阻断了下丘脑胰岛素信号, 导致葡萄糖不耐受和外周系统胰岛素敏感性。

Video correlati

Read Article