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Developmental Biology

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JoVE Journal Developmental Biology
Kwantitatieve geheel-mount immunofluorescentie analyse van cardiale Progenitor populaties in muis embryo 's
 
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Kwantitatieve geheel-mount immunofluorescentie analyse van cardiale Progenitor populaties in muis embryo 's

Article DOI: 10.3791/56446-v • 09:42 min • October 12th, 2017
October 12th, 2017 •
1,2,3, 4, 5,6, 1,2,3
1Cell, Developmental, and Regenerative Biology Department, Icahn School of Medicine at Mount Sinai, 2Mindich Child Health and Development Institute, Icahn School of Medicine at Mount Sinai, 3Black Family Stem Cell Institute, Icahn School of Medicine at Mount Sinai, 4Microscopy Core, Icahn School of Medicine at Mount Sinai, 5Department of Neuroscience, Icahn School of Medicine at Mount Sinai, 6Friedman Brain Institute, Icahn School of Medicine at Mount Sinai

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Hier beschrijven we een protocol voor hele mount immunofluorescentie en beeld-gebaseerde kwantitatieve volumetric analysis van vroege fase muis embryo's. Wij presenteren deze techniek als een krachtige aanpak van kwalitatief en kwantitatief cardiale structuren beoordelen tijdens de ontwikkeling, en voor te stellen dat het wellicht grote schaal aan te passen aan andere orgaansystemen.

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Ontwikkelingsbiologie kwestie 128 Confocal Microscopie beeldanalyse kwantitatieve beeldvorming organogenese hart ontwikkeling muis embryo
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