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Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle
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JoVE Journal Biochimica
Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

Improved Protocol for Chromatin Immunoprecipitation from Mouse Skeletal Muscle

DOI:
10.3791/56504-v

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09:30 min

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November 06, 2017

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, , ,
1Department of Functional Genomics and Cancer,Institut de Génétique et de Biologie Moléculaire et Cellulaire

Capitoli

  • 00:05Titolo
  • 00:59Isolation of Muscle Tissue and Tissue Lysis
  • 02:06Fixation and Nuclei Preparation
  • 04:04Assessing the Chromatin Fragment Size Following Sonication
  • 06:46Results: Purification of Nuclei from Adult Mouse Muscle
  • 08:09Conclusion

Summary

Traduzione automatica

Traduzione automatica

A novel protocol for the preparation of chromatin from adult mouse skeletal muscle adapted to the study of gene regulation in muscle fibers by chromatin immunoprecipitation is presented.

Tags

Chromatin ImmunoprecipitationMouse Skeletal MuscleGene RegulationChIP-grade ChromatinFormaldehyde FixationGlycine QuenchingNuclei IsolationCell Strainer FiltrationCentrifugation

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