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完整、全小鼠乳腺的分离及细胞外基质表达及腺体形态学分析

JoVE Journal
Biologia

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JoVE Journal Biologia

Isolation of Intact, Whole Mouse Mammary Glands for Analysis of Extracellular Matrix Expression and Gland Morphology

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完整、全小鼠乳腺的分离及细胞外基质表达及腺体形态学分析

DOI:

10.3791/56512-v

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12:49 min

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October 30, 2017

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Christopher Thompson, Katherine Keck, Abigail Hielscher

¹Department of Biochemistry and Molecular Biology,University of Nebraska Medical Center, ²Department of Biomedical Sciences,GA-PCOM

Capitoli

00:05Titolo
00:57Sample Procurement and Processing
02:29Immunohistochemistry
05:02Hematoxylin and Eosin Staining
06:19Ductal Analysis
08:33Immunohistochemictry Analysis
09:43Results: Whole Mouse Mammary Gland Isolation and Analysis
11:23Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个完整的, 完整的小鼠乳腺分离的协议, 以研究细胞外基质 (ECM) 的表达和导管形态学。小鼠 #4 腹腺从8-10 周大的雌性产妇小鼠中提取, 固定在中性缓冲福尔马林中, 用免疫组织化学方法对 ECM 蛋白进行切片和染色。

Tags

Mammary Gland Whole Mount Extracellular Matrix Gland Morphology Tumor Biology Stromal Remodeling Gene Expression Protein Expression Ductal Architecture Nulliparous Mice Formalin Fixation Paraffin Embedding Antigen Retrieval Immunohistochemistry

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