Immunofluorescence Microscopy of γH2AX and 53BP1 for Analyzing the Formation and Repair of DNA Double-strand Breaks

Journal / Cancer Research /

JoVE Journal
Cancer Research

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JoVE Journal Cancer Research

Article DOI: 10.3791/56617-v • 10:47 min • November 3rd, 2017

November 3rd, 2017 •

This manuscript provides a protocol for the analysis of DNA double-strand breaks by immunofluorescence microscopy of γH2AX and 53BP1.

Immunofluorescence Microscopy &947;H2AX 53BP1 DNA Double-strand Breaks Formation Of DNA Breaks Repair Of DNA Breaks Gamma-H2AX 53BP1 Immunofluorescence Microscopy Cancer Research Genetic Instability Visualizing DNA Breaks Repair Processes Susanne Brendel Laboratory Technician Anti-coagulant Stock Solution Heparin Sodium Chloride Collection Tubes 10X Lysis Solution Red Cells Ammonium Chloride Ammonium Bicarbonate Ethylenediamenetetracedic Acid Solution PH 8.0 Fixation Solution Potassium Hydroxide Solution Paraformaldehyde (PFA) Microtiter Tube Phosphate Buffered Saline (PBS)