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拟南芥线粒体的分离和呼吸测量
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Biochimica
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JoVE Journal Biochimica
Isolation and Respiratory Measurements of Mitochondria from Arabidopsis thaliana
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拟南芥线粒体的分离和呼吸测量

DOI:
10.3791/56627-v

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09:54 min

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January 05, 2018

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, , , , , ,
1ARC Centre of Excellence in Plant Energy Biology, Department of Animal, Plant and Soil Science, School of Life Science,La Trobe University, 2School of Biological Sciences,Flinders University, 3ARC Centre of Excellence in Plant Energy Biology,University of Western Australia

Capitoli

  • 00:05Titolo
  • 00:50Preparation of Grinding Medium, Wash Buffer, and Gradient Solutions
  • 02:22Homogenization and Mitochondrial Isolation
  • 06:30Oxygen Consumption Measuurements
  • 08:42Results: Isolation of Purified Mitochondrial Protein and Oxygen Consumption Measurement of Purified Mitochondria Obtained from Arabidopsis thaliana Plants
  • 09:37Conclusion

Summary

Traduzione automatica

Traduzione automatica

由于线粒体只占植物细胞的一小部分, 它们需要被纯化以进行一系列的研究。线粒体可以通过同质化的各种植物器官分离, 其次是微分和密度梯度离心, 以获得高纯度的线粒体分数。

Tags

Mitochondria IsolationArabidopsis ThalianaRespirationMitochondrial CompositionBiogenesisMetabolismEnergy BiologyStress ToleranceGradient CentrifugationPVP GradientGrindingFiltration

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