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Developmental Biology

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JoVE Journal Developmental Biology
Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice
 

Isolation, Culture, and Differentiation of Bone Marrow Stromal Cells and Osteoclast Progenitors from Mice

Article DOI: 10.3791/56750-v • 08:07 min • January 6th, 2018
January 6th, 2018 •
1, 1, 1, 1
1Maine Medical Center Research Institute

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In this article, we present methods to isolate and differentiate bone marrow stromal cells and hematopoietic stem cells from mouse long bones. Two different protocols are presented yielding different cell populations suitable for expansion and differentiation into osteoblasts, adipocytes, and osteoclasts.

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Isolation Culture Bone Marrow Stromal Cells Osteoclast Progenitors Mice Technique Bone Harvest Procedure Stem Cell Culture Medium Dissection Steps 78-week-old Euthanized Mouse Supine Position Dissection Board 70% Ethanol Forceps Skin Incision Iliac Crest Femur Heads Pelvis Ankle Joints Tibia Knee Joint
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