Rivista
/
Neuroscienze
/
体内荧光记者对小鼠视交叉上细胞核生物钟基因表达的监测
JoVE Journal
Neuroscienze
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Neuroscienze
In Vivo Monitoring of Circadian Clock Gene Expression in the Mouse Suprachiasmatic Nucleus Using Fluorescence Reporters
Please note that all translations are automatically generated. Click here for the English version.

体内荧光记者对小鼠视交叉上细胞核生物钟基因表达的监测

DOI:
10.3791/56765-v

•

07:44 min

•

July 04, 2018

•

, ,
1PTN Joint Graduate Program, School of Life Sciences,Peking University, 2National Institute of Biological Sciences, Beijing

Capitoli

  • 00:04Titolo
  • 00:37Preparation of Optical Fiber
  • 01:14rAAV Cry1 Reporter Injection
  • 03:21Optic Fiber Insertion into the Adult Mouse SCN
  • 04:05In Vivo Fluorescence Signal Monitoring and Data Collection
  • 05:24Results: Flourescence In Vivo Recording in the SCN Under LD and DD Conditions
  • 06:54Conclusion

Summary

Traduzione automatica

Traduzione automatica

这种新开发的荧光技术能够长期监测自由运动小鼠视交叉上核 (SCN) 中昼夜节律基因的转录。

Tags

In Vivo MonitoringCircadian Clock Gene ExpressionMouse Suprachiasmatic NucleusFluorescence ReportersOptical FiberRecombinant AAVStereotaxic InjectionSCN

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

切片的制备，组织器官型培养，并记录在视交叉上核时钟基因活性的荧光素酶

脊髓电二：外吸电极的制作

小鼠视网膜神经节细胞的转染在体内电穿孔

总RNA的分离和下游下一代测序和基因表达谱的非激光捕获显微切割离散小鼠脑地区显微镜方法

高分辨率实时成像发展中的神经上皮细胞的行为

使用超声波血脑屏障破坏和锰增强MRI的脑功能成像

运行轮活动在啮齿类动物的昼夜节律的记录和分析

显微吗啉代寡核苷酸脑室微量注射成年斑马鱼大脑中的基因表达

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

基因表达的变化大鼠海马后，前丘脑核脑深部刺激分析

Read Article