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Neuroscience

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JoVE Journal Neuroscience
An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice
 

An Improved Method for Collection of Cerebrospinal Fluid from Anesthetized Mice

Article DOI: 10.3791/56774-v • 06:40 min • March 19th, 2018
March 19th, 2018 •
1,2, 3,4, 1, 5, 3,4,6, 1,4
1Shanghai Advanced Research Institute, University of Chinese Academy of Sciences, Chinese Academy of Science, 2Shanghai Institute of Materia Medica, University of Chinese Academy of Sciences, Chinese Academy of Science, 3Center of Functionally Integrative Neuroscience, Department of Clinical Medicine, Aarhus University, 4Sino-Danish Center for Education and Research (SDC), 5Department of Neurology, Xinhua Hospital Chongming Branch Affiliated to Shanghai Jiao Tong University School of Medicine, 6Department of Nuclear Medicine and PET-centre, Aarhus University Hospital

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This protocol describes an improved technique for the abundant collection of cerebrospinal fluid (CSF) with no contamination from blood. With greater sample collection and purity, more analyses can be performed using CSF to further our understanding of diseases that affect the brain and spinal cord.

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Keywords: Cerebrospinal Fluid CSF Collection Anesthetized Mice Micromanipulator Pulled Glass Capillary Capillary Holder Three-way Valve Syringe Surgical Procedure Stereotaxic Frame Cisterna Magna Dura Mater
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